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Nevertheless, we identified a large number of mutants unique to each condition.
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Though the absence of premeiotic S phase in msh5 mutants is apparently unique to C. cinerea, cell death is similar; msh5-22 cells die after arresting at metaphase I [ Figure 1 (Lu et al. 2003)].
To make sure that the observed GOF effects exhibited by the LS mutants were not unique to EGF signaling, we also examined hepatocyte growth factor (HGF -induced SHGF -inducedding and SHP2 Gab1t ERK1/2 activation in cells overexpressing wild-type and mutant SHP2.
The data for the alternate CLS screen with cells re-grown before Bar-seq analysis are provided in Table S5 (sequence counts per mutant in each sample) and in Table S6 (long-lived mutants unique for re-growth screen).
Next, we surveyed the identified commonly mutant genes in an additional cohort of 99 TCC patients by the conventional exome sequencing [ 6] (Additional file 9: Table S3), to determine whether these mutant genes were all unique to this patient or exist recurrently as mutant genes which would more likely to be candidate cancer genes.
More recently, technological breakthroughs have shown that numerous endogenous mutant cancer proteins are unique to tumor cells.
Moreover, wild-type Tau4R is as effective as mutant Tau.P301L which is unique, to our knowledge.
In order to address this question, we used a mutant of a subunit unique to the Rpd3S histone deacetylase complex, rco1Δ, which does not have HDAC function [5], [11].
Variants unique to mutant strains were identified using Select Variants (Depristo et al. 2011).
Certain mutant phenotypes that are unique to Drosophila and zebrafish do not correspond directly with those observed in humans, but they can nevertheless serve as useful quantitative assays for testing disease-associated mutations.
Candidate nucleotide differences for let-504 (h448 ) were further filtered to satisfy the following three criteria: (1) mutations that fall within genetic mapping range; (2) unique to mutant strain compared to the N2 strain (KR4848); and (3) allelic ratio falls between 60 and 90%.
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