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However, the thermal unfolding curves of V1A and V1D suggest that these mutants unfold completely at 75°C in contrast to R-BSX, which attains complete unfolding at 80°C.
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The V1G mutant unfolded almost completely at 60°C (Figure 4B), resulting in its slower migration compared to R-BSX.
Another significant change was observed when Val1 was changed to Leu; unfolding started at 70°C and the enzyme unfolded completely at 80°C (Figure 3A).
Unfold completely.
Overall protein stability was investigated by guanidine-induced unfolding, which showed less cooperativity for mutant unfolding, but with similar transition points of 4.5 M guanidine for the CXCL8 Δ6E70KN71K) mutant compared with 5 M guanidine for the WT (see Figure 3B).
The higher propensity of FALS mutants to unfolding as well as the different unfolding pathway may be involved in aggregation and pathogenesis of these mutants in ALS.
The F123W and S108L mutants both unfolded at lower pressures, while retaining some resistance to pressures below 50 MPa.
Over the next several months as the ordeal unfolded, we completely embraced her.
In case of Tm1 < T < Tm2 (Tm3) only the CH2 domain <span class="lh lhl">unfolds but completely refolds by restoring room temperature.
In contrast to ClpX, however, Pch2/TRIP13 does not completely unfold its HORMA domain protein substrates.
Note that you should not unfold the model completely.
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