In the presence of PLP, the apomonomer of the triple mutant undergoes a biphasic process: the fast phase represents the formation of an inactive PLP-bound monomer, while the slow phase depicts the monomer-monomer association that parallels the regain of transaminase activity.
However, a previous study showed that the Alab49 ΔfctA mutant undergoes a sharp reduction in the amount of pilus-associated proteins present in a polymeric form, yet the ΔfctA mutant behaves similar to wt Alab49 in terms of net growth at the skin at 7 d post-inoculation [17].
The ced-9 loss-of-function mutants undergo inappropriate apoptosis in cells that are normally destined to survive, whereas a ced-9 gain-of-function mutant is defective for apoptosis induction in cells normally destined to die.
COP1 is a major negative regulator of photomorphogenic responses, so that cop1 mutants undergo photomorphogenesis in darkness in the absence of photoreceptor activation (Deng et al, 1991).
Results showed that overexpression of wild type PCMT renders cells resistant to apoptosis, while a high percentage of cells transfected with antisense or either negative mutants undergo apoptosis upon H2O2 treatment.
A question left unanswered in these studies is whether spindle checkpoint mutants undergo genetic alterations other than aneuploidy.
There are even some HAE HSL2-independent hydrolases, particularly XTHs, which may explain the observation that hae hsl2 and ida mutants initially undergo a decrease in the force required to remove the floral organs before increasing again [ 6].
There was a reduced level of total protein, and Rec8-GFP persisted even after 24 hours post meiotic induction, suggesting that the inability of haploid pat1 dfp1-r35 mutants to undergo a second meiotic division is due to the persistence of Rec8 in this strain.
