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In conclusion, the genetic results revealed that the gTME technique could be an effective approach for construction of novel mutants under various external stresses.
RbcL was frequently found to be differentially expressed in wild type and microcystin-deficient mutants under various conditions tested (Table 1 and unpublished data).
Future experiments will determine if increased cleaved tRNA fragments are observed in the RMTase mutants and testing these mutants under various environmental conditions may highlight additional roles for these genes in modulating stress responses.
Recently a genetically encoded nanosensor for Arg was developed, that can be used to report cytosolic, mitochondrial or plastidic Arg levels in wildtype and oat mutants under various nutrition regimes [ 43].
However, the availability of a genome-wide collection of deletion mutants, as well as tag arrays for quantifying the proportions of individual mutants in a population, has enabled genome-wide screening for phenotypes of S. cerevisiae deletion mutants under various conditions ([ 5, 33, 4]).
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Comparisons in the cellulase activity between the wild type as the control and the mutant under various buffer pH values and temperatures showed that the cellulase activities of the mutant were clearly higher than that of the control, particularly for neutral pH and lower and higher temperatures.
In this regard, the collection of 5000 yeast gene deletion strains provides a unique resource for systematic analysis of gene interactions by comparing cell proliferation phenotypes (CPPs) of the WT strain and each deletion mutant under various perturbation conditions [ 2- 4, 8, 9].
Mutation experiments (M E) measure the fitness of a set of independently-derived single step mutants evaluated under various environments and provide with direct information on the distribution of mutational effects.
To test whether other mutants with unstable genomes but still in possession of their mitochondrial DNA behave likewise, we measured CIN in a few selected RHO+ cell-cycle mutants grown under various conditions shown to affect rho0 cells (MCR, in the presence of ethanol or antioxidant Tiron).
We next characterized the phenotypes and molecular properties of the mutant strain under various in vitro conditions.
(C ) Example transcription trajectories of the E1103G mutant Pol II under various conditions.
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clones under various
genes under various
mutants under long
mutants under DTT-induced
mutants under aerobic
mutants under restrictive
mutants under normal
mutants under basal
mutants under native
mutants under anaerobic
mutants under non-mating
mutants under isotonic
mutants under low
mutants under mock
mutants under high
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