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One was "X2," the "X-Men" sequel about Wolverine, Storm, Rogue and the other superpowered mutants under the tutelage of Professor X taking on the forces of evil and intolerance.
For this, the phenotypic susceptibility assays were done with various such mutants under the similar conditions which required functional calcineurin.
No apparent visual phenotype was observed in any of the single knockout mutants under the conditions tested.
Interestingly, several pupylation targets did not show increased steady state levels in degradation-defective mutants under the conditions tested.
We have combined these mutants under the name Salsa (Table 1 and Fig. 2) and are still in the progress of identifying the causative mutation.
The sensitivity to ethylene could be restored when the GFP- and TAP-tagged AHK5 fusion constructs were expressed in the ahk5 mutants under the control of the 35S promoter (Fig. 5C).
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First, like eif3h mutants, rpl24b mutants under-translate the AtbZip11 mRNA, and the detailed spectrum of translational defects in rpl24b is remarkably similar to that of eif3h.
No labeling was observed in an Atcrt1b mutant under the same conditions (data not shown).
To verify its sub-cellular localization, CPR5 was fused (C-terminal) with EGFP and permanently expressed in cpr5-1 munder under the control of the CaMV 35S promoter.
A noticeable fraction of the ΔrovA mutant cells appeared to be lysed, suggesting that the bacterial membranes might be constructed differently, thus unstable in the ΔrovA mutant under the experimental conditions employed, leading to the disruption of the bacterial cells.
The results showed no visible difference between the wild type and the ∆ slr1037 mutant under the tested stress conditions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com