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Therefore, we compared temperature-dependent hyphal induction in SJ chk1- hyp mutants under several nutrient conditions.
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To determine whether the ∆ slr1037 mutant was sensitive specifically to butanol, experiments were conducted to compare growth patterns of the wild-type strain and the ∆ slr1037 mutant under several other stress conditions: 1.75% ethanol (v/ v), 4% NaCl (w/ v), pH 6.5, pH 11.0, and 8 mM urea.
Mutant age3Δ cells have a severe defect in filament formation under several different hyphal growth conditions.
Interestingly, the ∆ slr1037 mutant grew similarly to the wild type under several other stress conditions tested, which suggests that its regulation on butanol tolerance is specific.
The aisles had once swarmed with people, mutant under-dwellers who emerged from the depths of Penn Station.
Detailed phenotypic characterization of cin mutants revealed several other phenotypes similar to those of tub mutants.
Our screening of transposon mutants identified several additional attenuated strains.
Working with these mutants has several benefits.
Interestingly, several pupylation targets did not show increased steady state levels in degradation-defective mutants under the conditions tested.
This is supported firstly by the decreased growth rates of the TS-clathrin mutant over several days at the non-permissive temperature underlining that clathrin function is severely restrained under these conditions.
To examine the relationship between the defective seed phenotypes and yield components in the z3 mutant, we evaluated several agronomic traits under NLD conditions: main panicle length, number of panicles per plant, number of spikelets per main panicle, seed setting rate, 500-grain weight, and grain yield per plant (Additional file 2: figure S2).
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