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In summary, our gene expression analyses have demonstrated that genes involved in sexual development are de-repressed in the ssn8 mutants under non-mating and mating conditions.
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Furthermore, we showed that this strain did not yield any escape mutants under non-permissive growth conditions with an assay detection limit of 1.9 × 10−13 per CFU, which is lower than that of any other reported biocontainment strategies.
fri FLC plants flower slightly later than fri flc mutants under non-inductive photoperiods, indicating FLC can function independently of FRI in the floral pathway [ 17].
The males perform their excretory alchemy in both the mating and non-mating seasons.
Non-mating flies were removed and mating pairs were left until the next day.
Thus, dopaminergic function could be selectively enhanced during mating periods, and may decrease during non-mating intervals.
Our conservative analysis excluded any non-mating pairs, where our complete analysis reflected the latency to mating as 90 min.
RESULTS: A non-mating OVX model with satisfactory implantation rates was established.
Copulation duration calculations exclude data from non-mating pairs.
Data for non-mating pairs was excluded from this statistical analysis.
Data for non-mating pairs was excluded from all statistical analyses (except see Results).
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