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However, strong reversible self-association observed for selected mutants under native conditions did not correlate with aggregate growth, indicating 'sticky' surfaces that are exposed in the native monomeric state are inaccessible when aggregates grow.
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Ectopic scc1 mutants under its native promoter were incorporated at the leu2 locus in cells whose endogenous wild type gene SCC1 is under the control of Gal1 10 promoter.
We also carried out limited proteolysis of BSX and all its mutant proteins with trypsin under native conditions, and the peptide mass fingerprinting was obtained using mass spectrometry.
RMY102 was transformed with a second CEN plasmid marked with TRP1 carrying WT H3 (HHT2) and either a WT or a mutant copy of H4 (HHF2), expressed under native promoter control.
We transgenically expressed WT AGO18, YF/AA, and D833A mutants under the control of the native AGO18 promoter in the ago18 mutant background.
The aisles had once swarmed with people, mutant under-dwellers who emerged from the depths of Penn Station.
The wild type AdoMetDC and mutants were purified using a Ni-NTA (Qiagen) affinity column under native conditions in the presence of 2.5 mM putrescine.
Certain programs may only run under native 32-bit environments.
Activity bands were excised, loaded immediately into the Native gel, and then electrophoresed under Native conditions.
Native BSX was found to exhibit considerable resistance against proteolytic digestion with trypsin under native conditions.
Under native conditions, wild type p66 subunit formed homodimer.
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