For instance, the mutants unable to produce trehalose (i.e., the Δtps1Δtps2 and Δtps1Δhxk2 mutants) were more sensitive to osmotic stress than the wild-type strain (Hounsa et al. 1998).
In this work, we examined a rugose strain of V. cholerae and its mutants unable to produce matrix proteins by interfacial rheology to compare the evolution of pellicle elasticity in real time to understand the molecular basis of matrix protein contributions to pellicle integrity and elasticity.
This finding is consistent with previous studies on mutants unable to utilize C1 and C2 substrates [17], [26], [38], [59] and with a very recent study [44].
viciae mutants unable to transport branched-chain amino acids via the two main amino acid ABC transport complexes AapJQMP and BraDEFGC produce a nitrogen starvation phenotype when inoculated on pea (Pisum sativum) plants [1], [2].
This is in sharp contrast to mutants unable to synthesize bacillibactin, the other siderophore produced by B. anthracis, which grows well in IDM and shows no significant attenuation in a mouse model of infection [15].
Cdc24 mutants unable to bind to Far1 are constitutively located in the cytoplasm; however, Cdc28 activity is still required to activate these cytoplasmic mutants, indicating that nuclear export of Cdc24 is not sufficient to promote GEF activity [11], [12].
The analysis of sporozoite invasion of hepatocytes in vitro as well as development within primary human hepatocytes with P. falciparum Δp52 mutants demonstrates a pattern of attenuation essentially identical to P. berghei mutants unable to express P36P.
However, Lidke et al. (unpublished data of our laboratory) have determined that ERK mutants unable to dimerize retain the ability to translocate to the nucleus, albeit at a slower rate.
Whereas, early on the investigative timeline, B. japonicum single mutants unable to be cultured autotrophically on exogenous H2 yet still able to recycle endogenous H2 in soy nodules were identified [26].
According to this idea, PS1 mutants unable to generate CTF2 but still capable of being processed, may behave less severely than those deficient in processing, since their effect on β-catenin·Tcf-4 transcription would be less robust.
Significantly, repeated wounding did not stunt the growth of the leaves of mutants unable to synthesise JA, or unable to respond to JA including coi1, jai3, myc2, but not jar1.
