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An aggravating factor to the misfolding of p53 caused by single amino acid mutations is the negative dominance property: several p53 mutants (translated from a single mutant allele) are able to drive wild-type p53 protein (translated from the remaining wild-type p53 allele) into a mutant conformation, 46) in a way that resembles the action of the prion protein.
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Wild type (WT) and mutant SF1 proteins were in-vitro translated from the pCMX expression vector using the TNT reticulocyte system (Promega, Southampton, UK).
Thus, the CDC2 and FPK polypeptide pieces translated from the wp mutant allele may have potential to interfere with the functional enzymes translated from intact CDC2 or FPK host genes.
The above results are consistent with reaper and hid being translated from the upregulated reaper and hid transcripts in pcm mutants.
Answers translated, from the Japanese, by Ted Goossen.
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