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Increased metalloproteinase activity in color mutants may contribute to the augmented ability of color mutants to induce aggravated immune responses from infected larvae.
Increasing levels of Abl activity also interact with heterozygous robo mutants to induce ectopic crossovers, and overexpression of Abl in a comm mutant, experiencing high levels of midline repulsion, actually improves commissure formation [49].
On the other hand, the ability of NGFR100 mutants to induce pain sensitization in animal models was markedly reduced, correlating well with the loss of pain perception in HSAN V patients.
Interestingly, the virulence of the mutants to induce abscess in mice can be restored by co-application of the variants with the purified EPS [ 7].
The failure of pUL31 mutants to induce vesicle formation in pUL34 GUVs could be caused by defects in their membrane remodeling activity and/or in their ability to bind pUL34, possibly as a result of severe misfolding of the protein.
SOS phenotypes were determined using a high-throughput β-galactosidase assay in microtiter plates, measuring both constitutive SOS levels and the ability of the mutants to induce SOS following nalidixic acid treatment.
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To this end, we show that in Whitlock-Witte B-cell transformation assays the ability of the triple mutant to induce outgrowth is completely abrogated.
Given these observations, and the retained ability of the Schu S4 ΔpurMCD mutant to induce tissue damage in the lungs following i.n.n
To assess the ability of the triple mutant to induce a myeloproliferative disease in vivo, lethally irradiated Balb/c mice were transplanted with bone marrow transduced with wild type, triple mutant or empty vector.
In their original publication, Berndt and colleagues used the C128A mutant to induce long and reversible subthreshold depolarizations in dissociated neurons and to sensitize cells to synaptic input [6].
In fact, the only other mutant to induce less ISG15 production was the ΔactA mutant.
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