Sentence examples for mutants to generate from inspiring English sources
In addition, we analyzed the ability of mutants to generate protein-DNA complexes with specific target DNA attP.
The inhibition of Bax-mediated condensed chromatin depended on the ability of the mutants to generate cytosolic PrP.
The resulting vectors pRH001-ptsP and -ptsO, -ptsOH30A and -ptsOS61A (Table S1) were transferred by mating into the ΔptsP or ΔptsO mutants to generate the complemented strains ΔptsP/ptsP, ΔptsO/ptsO, ΔptsO//ptsOH30A and ΔptsO/ptsOS61A.
To uncover possible redundancies with other AP2Cs that we have shown above to interact with MAPKs we crossed ap2c3 with T-DNA insertion lines ap2c1 [26], ap2c2 and ap2c4 mutants to generate double mutants (Figure S4).
We also show in this paper that the loss of GSS mutants in inhibiting Bax-mediated condensed chromatin results from the inability of these mutants to generate CyPrP in MCF-7 cells.
Helix-forming propensities were averaged across all the fifty mutants to generate a single value representing the mutated mRNA helix-forming propensity.
As an alternative, we made use of the temperature sensitive cdc16-116 mutont to generate a high number of cells lacking nuclei and SPBs.
Therefore, we assessed the ability of C. jejuni Δppk2 mutant to generate GTP from poly P. The C. jejuni Δppk2 mutant was significantly (P≤0.05) defective in poly P-dependent GTP generation compared to WT (Figure 1A), while complementation restored the ability of the Δppk2 mutant to generate GTP to levels comparable to WT (Figure 1A).
In order to delete the remaining Fas2 gene and reuse the disruption construct, the SAT1 flipper cassette containing the selection marker was eliminated in the selected mutant to generate the sensitive heterozygous mutant (Figure 1, lane 3).
The vte1 npq1 mutant was crossed with the pdx1 single mutant to generate a triple mutant (vte1 npq1 pdeficientcinnt in vitamins E and B6 and in zeaxanthin.
The let-7a miRE or its mutant in PTC102-L7 or PTC102-L7M was replaced with a miR-21 miRE or its mutant to generate PTC102-21 and PTC102-21M.
