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Use of gibberellin (GA) biosynthesis mutants and BR signaling mutants to analyze the mechanism of action of this synthetic series indicated that the primary site of action is BR biosynthesis.
Using live single cell imaging, we were able to use these mutants to analyze functions of the C-terminus in chemokine-induced Ca2+ responses and heterodimer formation.
Dai and Pendergast [ 2] used a series of deletion mutants to analyze Abi-2 regions that are involved in the interaction with c-Abl.
Mutants for seven genes were not available (see the section Materials and Methods), leaving us with 36 viable mutants to analyze.
Root hair deformation assays and infection with S. meliloti was carried out on these mutants to analyze the response of root hairs to bacterial Nod factor (NF) and the induction of cortical cell division following rhizobial inoculation.
We then used these mutants to analyze the contribution of CipA and its functional modules (CohI, CBM, and XDocII) and secondary scaffoldins and their SLH modules to cellulose degradation.
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In this work we used the previously described non-toxic Cry1Ab-D136N/T143D mutont to analyze if its DN phenotype extends to other Cry1 toxins.
Because SEC6 is essential in S. cerevisiae, we constructed a conditional mutant to analyze gene function in C. albicans.
SHP2 activity in oral cancer cells was reduced using si-RNA knockdown or enforced expression of a catalytically deficient mutant to analyze migratory and invasive ability in vitro and metastasis toward the lung in mice in vivo.
Protein blocks approach has also been used to build trans-membrane protein structures [42], to design peptides [48], to define reduced alphabets for designing mutants [49], to analyze protein contacts [50], to find structural motifs across protein families [18] and to identify Mg2+ binding sites in proteins [51].
We generated Venus-transgenic and T21D12.3-mutant nematodes to analyze developmental expression patterns and in vivo functions of the nematode PQBP1 homologue protein (pqbp-1.1).
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