Sentence examples for mutants through the from inspiring English sources
The 35S:Z3 construct in the pMDC32 vector was introduced into calli generated from mature seed embryos of z3 mutants through the Agrobacterium (strain EHA105 -mediated mEHA105 -mediatedl. 1999; Lee et al. 1999).
We took advantage of the targeted knockout technique to generate dRecQ4 mutants through the replacement of the endogenous locus with an engineered mutant form via homologous recombination.
Because Cas9D10A can efficiently generate indel mutants through the NHEJ pathway, we then evaluated the efficiency of Cas9D10A in HDR.
Elena et al. 23 used untargeted mutagenesis to generate a set of Escherichia coli mutants through the random insertion of transposon (Tn) elements throughout the genome.
However, phenotypes were revealed for these two mutants through the chemical sensitivity assays, resulting in at least one phenotype for every phosphatase mutant analyzed.
2) The absence of Rh1 apoprotein can be explained by the degradation of misfolded Rh1 in the EMC null mutants through the accelerated ERAD pathway.
NMR experiments have also been used to assess water accessibility of the cluster and its mutants through analyzing the H2O/D2O exchange rates.
To determine whether MoSec22 could complement Sec22 function, we expressed MoSEC22 in a Δsec22 mutant through the yeast expression vector pYES2.
To genetically model the predominant ribosome defect in DBA, we generated an rps19 null mutant through the use of TALEN-mediated gene targeting in zebrafish.
In order to verify that the fluorescein molecule is covalently bound to the Cys residue in the V216C mutant through the maleimide linker, a control experiment was performed in which the V216C mutant was incubated with fluorescein (without the maleimide linker) and then analysed by SDS/PAGE.
We generated stronger γCOP mutants through remobilization of the P{lArB}A383.2M3 element, which is inserted within the 5'UTR of the γCOP transcription unit ([33]; Figure 1A, E; Supporting Information Text S1).
