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In this study, we examined the activities of 20 actKR mutants through a combination of kinetic studies, PKS reconstitution, and structural analyses.
The notion was to attempt to enrich for possible mutants through a fat body insertional enhancer trap screen followed by a comprehensive longevity screen.
DOI: http://dx.doi.org/10.7554/eLife.01473.005 > -wrap-foot> We identified the sleep-altering lesion in rye mutants through a combination of recombination mapping and deep-sequencing.
Cell viability in HEK293 cultures was assessed after 72 96 h of overexpression of RUNX2-GFP or of its mutants through a colorimetric reaction, by measuring the cellular reduction of 3- 4,5-Dimethylthiazol-2-yl -2,5-Diphenyltetrazolium Bromide (MTT; Molecular Probes-Invitrogen) to formazan (44).
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The soluble oligomeric species, formed by the apo form of both WT SOD1 and its mutants through an oxidative process, might represent the precursor toxic species, whose existence would also suggest a common mechanism for ALS and fALS.
However, the H3 triple mutant may also have arise from H2 double mutants through an additional mutation occurring at position 59 of the dhfr gene.
A previous study in yeast had also identified a tah18 mutant through a genetic screen for conditional tah top1T722A-hypersensitiveexhibiting exhibithermo-sensitiveitive growth at 36°C in the presence of the camptothecin mimetic mutant top1T722A [25], [26].
The screen identified a muscle mutant through a highly significant reduction in birefringence, and SNP-based linkage analysis led to the identification of a nonsense mutation in tmod4.
The first evidence of biological and physiological function of CAMTA protein was recently reported in Arabidopsis CAMTA3 (AtSR1) loss of function mutant through a reverse genetic approach [ 18].
The proportion of light output above λ=600 nm is a key determinant for transmission efficiency through mammalian tissue.[ 39] Bioluminescence data were acquired using different band-pass (bp) filters in a Photon Imager and showed that most light from the x5 Fluc mutant with 6 was collected through a λ=670 720 nm bp filter and from the x5 S284T mutant through a λ=700 750 nm bp filter.
The expression of T3SS was upregulated in the ΔrovA mutant through an indirect regulatory mechanism, which is possibly related to the altered membrane construction in the mutant.
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