Exact(1)
RAD1 was originally identified in S. cerevisiae in a screen for mutants that rendered cells hypersensitive to ultraviolet (UV) radiation (Cox and Parry 1968).
Similar(7)
As an alternative approach, we exploited the strong conservation between CREB and ATF1 in the KID region and constructed an ATF1D39E,E41D mutant that rendered the ATF1 ATM/CK cluster identical to its CREB counterpart, which was readily detected using an α-pCREB-108/111/114 CREB antibody (Fig. S1B).
The two temperature-resistant mutants seem to carry suppressor mutations that rendered cells temperature-resistance and abolished L-glutamate overproduction.
Uninspired plotting lands him in mutant prison, shackled by a techno-collar that renders his mutant powers useless and brings back his cancer, which, in turn, allows for some impressively morbid fight scenes featuring body-twisting physical humor.
Consistently, SpTORC2-Gad8 mutants were synthetic lethal with loss of either S. pombe Rad52 or Rad51, possibly indicating that SpTORC2-Gad8 mutants incur an enhanced level of DNA damage that renders them dependent on HR.
The TAT-STEP46 was affinity purified as described by Paul et al (2007); it has a point mutation in its catalytic domain (C300S) that renders it enzymatically inactive, and thus acts as a substrate-trapping mutant.
That renders the virus less lethal.
E. coli populations grown in lactose-limited chemostats, for example, are rapidly dominated by mutants that render the lac operon constitutive and thereby express high levels of the lactose:proton antiporter encoded by lacY.
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