Consequently, we obtained a mutant that provides higher product yields than the conventional Taq pol without decreased fidelity.
Without being aware of the nature of the genetic alterations in their "optimized" strains, BW workers at Camp Detrick selected a mutant that provided dramatically higher total and relative spore yields, and generated consistent experimental results [6].
Therefore we created chimeric fish with mutant xanthophores next to wild-type melanophores by transplanting cells from leo tK3 ;spa rse mutant blastula embryos (that provide mutant xanthophores [ Parichy et al., 1999]) into pfe hosts (lacking xanthophores).
The new data are consistent with the original model and provide new mutants that allow one to ascribe certain features to the linker, and not decoupling.
Replicative fitness relates to an optimal mutant spectrum that provides the molecular basis for phenotypic flexibility, with implications for antiviral therapy.
The 2000 cichlid species in these lakes represent a collection of natural mutants that may provide insight into the genetic mechanisms of speciation and adaptation [ 7].
Besides the C-Jun and AtBPC6 zippers, we also modeled two mutant domain versions that provided us with more insight on the function of the alanines inside the zipper: In C-Jun-ALA the leucines that contribute to the zipper were exchanged for alanines, while in BPC6-LEU the conserved alanines inside the homology modeled AtBPC6 domain were replaced by leucines.
We characterize a mutant of HPr that provides relief from CCR and we discuss the possible mechanism by which the mutation leads to the observed growth characteristics.
In this situation, a mutant self-compatible hermaphrodite that provided reproductive assurance when pollination agents were a limiting factor, and could outcross with other hermaphrodites, should have been favoured over a female-sterile hermaphrodite (pollen-only) [ 17, 27, 68].
Genotype stability and phenotype hereditability of the mutants can be maintained at any stage of evolution when the genetically modified proofreading elements are removed from the evolved mutants, and that provides great convenience for further genetic manipulation and application of mutants, e.g. introduction of the metabolic pathway for products synthesis.
