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Such mutants can be functionally distinct from mutants that maintain the integrity of the ATP binding site [ 42].
Host diversification selects for phage mutants that maintain infectivity by increasing genetic similarity to dominant bacterial strains.
Selection for mutants that maintain mtDNA when mitochondrial fusion is blocked has successfully revealed mitochondrial division machinery components (Fekkes et al. 2000; Mozdy et al. 2000; Tieu and Nunnari 2000).
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Eight NBD1/linker and eight NBD2 mutants that maintained the highest levels of resistance against all three fungicides (highlighted in Figure 5A) were chosen to be randomly assembled into mutants with all six intracellular Cys removed (C6Z).
HCCS mutants that maintained synthetase activity (i.e., attached heme and released the 12 kDa holocytochrome c product) yielded holocytochrome c at levels comparable to or better than wild type (WT) HCCS; mutants with functional defects released significantly less holocytochrome c.
Using this method, we found that the U5-IR stem library is rapidly enriched for mutant viruses that maintain the proper RNA structure of the region.
Therefore the isolation of mutants of a transporter that maintain the structure of the wild type protein and yet are conformationally restrained would greatly facilitate structure determination.
Studies with deficiency mutants have identified multiple systems that maintain DNA methylation in Arabidopsis [35] [38].
Reconstruction of the G helix using site-directed, saturation mutagenesis yielded a mutant, N403P/L405H, that maintained its specific activity and showed higher selectivity to germacrene D-4-ol in vivo (up to 93%).
Furthermore, unlike aPKC and DE-cad, aPKC and Crb localization appeared to be linked as all mutant cells that maintained apical Crb also maintained apical aPKC, while all those that showed a loss of apical Crb also showed a concomitant loss of aPKC.
The signaling pathways that maintain hepatic homeostasis are highly conserved, as demonstrated by studies of the hi559 zebrafish mutant.
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