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Mutants that reduce neurotransmitter release are resistant to the effects of aldicarb, whereas mutants that increase neurotransmitter release are hypersensitive.
This finding suggested an efficient method of isolating mutants that increase synaptic transmission.
We previously used the ade2 -min3 reporter construct to identify mutants that increase stationary-phase minisatellite instability.
C. elegans mutants that increase sperm number, for instance, were shown to be of disadvantage under natural conditions because the additional spermatogenesis delays oogenesis and therefore the minimum generation time [ 62].
Such regulators can be easily identified in live animals as mutants that increase the abundance and/or apical membrane localization of the PGP-3ΔF508 PGP-3ΔF508s measured by fluorescence.
It has been shown that Notch activation is greatly reduced in mutants that block entry into the early endosome but is enhanced in mutants that increase endosomal retention [ 44].
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We identified 28 F. novicida transposon mutants that increased the rate of cell death of infected macrophages.
We utilized the ade2 -min3 reporter in a screen for mutants that increased the frequency of minisatellite alterations (Kelly et al. 2007).
However, another factor is that the N-WASP diVCA construct we use was deliberately chosen because it is only a weak activator (this helped us to engineer mutants that increased activation).
However, the few mutants that increased over the time course relative to the reference timepoint, and therefore have longer lifespans than the pool average, are more likely to directly interfere with the aging process.
In the first approach, I performed mutagenesis studies to engineer a yCD mutant that increases the sensitivity of E. coli and mammalian cells to 5-fluorocytosine.
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