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This may be due to the high mTOR activity in the mutants that could not be fully blocked by rapamycin treatment under these experimental conditions.
This required isolation and genetic mapping of mutants that could not grow without methionine, leading to an interest in the mechanism of recombination, the process that ensures that new combinations of genetic variants are transmitted from one generation to the next.
From this screening, we isolated 24 suppressor mutants that could grow on 5-FOA medium.
With reckless ambition, I decided to add this fusion back into cells and then screen for mutants that could not either switch on or switch off the response to heat shock.
Various characters were included at the suggestion of editor Mark Helfrich, who brought to director Brett Ratner Marvel's X-Men Encyclopedia searching for mutants that could make appearances.
Similar observations were made with NTS1 directly after solubilization [16] and with structurally unstable constitutively active receptor mutants that could be stabilized by agonist addition [17] [19].
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So I took a yeast mutant that couldn't divide because it was defective in the controlling gene.
In this study, we attempted to create a lysine-deficient LIGHT mutant that could be PEGylated site-specifically and would have lower affinity for DcR3.
Under low-nutrient conditions and treatment with the pathogenic oomycete, Hyaloperonospora parasitica, wild type had a higher fitness than the mutant that could not activate SAR, demonstrating that normal inducible SAR is beneficial in these conditions; this result, however, was not found under high-nutrient conditions.
A Zfp277 mutant that could not bind Bmi1 did not rescue Zfp277−/− MEFs from premature senescence.
In order to further understand the regulation of WRN acetylation and its stability, we identified WRN acetylation sites and made a WRN mutant that could not be acetylated to test its stability.
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CEO of Professional Science Editing for Scientists @ prosciediting.com