Sentence examples for mutants that contained from inspiring English sources
To directly test this hypothesis, we engineered GluN2B mutants that contained phenylalanine in place of tyrosine at three different sites and transiently expressed them with the GluN1 subunit in human embryonic kidney (HEK) cells.
Here, we present the design and structural studies of the monoclonal antibody mAb15 and related mutants that contained a series of progressively germline mutations only in the heavy chain variable domain (VH) that ultimately led to an increase of more than 11 °C in the melting temperature (Tm) of the antigen-binding fragment (Fab).
Also, lin-10 mutants that contained elevated levels of PMK-3 did not demonstrate the dramatic enhancement observed in rpm-1 lin-10 double mutants (Fig. 4E,J).
Moreover, lin-10 mutants that contained elevated levels of DLK-1 demonstrated a dramatic enhancement in phenotype (Fig. 4F,J), similar to that observed in rpm-1 lin-10 double mutants.
Only the p22 deletion mutants that contained amino acids 103 148 were present in the membranous fraction of cells, including a construct encoding amino acids 103 148 alone; all constructs that did not contain amino acids 103 148 were present in the cytosolic fraction.
Moreover double mutants that contained bulky Cys63 substitutions and a mutation in the peptide-binding domain of Kar2 no longer protected cells to oxidative stress.
Using a pool of VPE mutants that contains every possible combinations of the 20 canonical amino acids, specific binders could be searched empirically [1, 3].
In vitro experiments were conducted with a pool of mutants that contains a mutant with an attenuated growth on rich medium.
A band corresponding to a dimer of MreB occurs with mutants that contain a cysteine in the dimer interface (indicated in bright colours).
Several Apaf-1 deletion mutants that contain C-terminal His-tags were designed and constructed by referencing the previous study [ 29].
However, the difficulty of detecting mutants that contain deletions of homoeologues of target genes has been a major factor limiting the application of this method to polyploid species.
