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Hypotonicity triggered on average a 130% increase in the currents from mutants that cause the mildest form, BO (Fig. 3d).
Furthermore, expression of PrP mutants that cause hereditary Creutzfeldt-Jakob disease or fatal familial insomnia led to accumulation of PrPRES and their partial retention at the ER, associated with a drastic decrease of ER calcium content and higher susceptibility to ER stress.
The importance of GnRHR compartmentalization is best illustrated by point mutants that cause hypogonadotropic hypogonadism.
Distinct from these approaches, the Rnr3 R-SGA identifies mutants that cause induction of the DDR.
Better correctors are needed to repair cystic fibrosis transmembrane conductance regulator (CFTR) processing mutants that cause cystic fibrosis.
These findings will contribute to our understanding of naturally occuring dominant-negative STAT3 mutants that cause the hyper-IgE syndrome.
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The GSK responses of the mutants that caused the mildest forms of MD were statistically indistinguishable from those that caused SMDK (Fig. 4d).
Although rpm-1 mutants are reported to move normally, rpm-1 (lf) has a synthetic synaptic phenotype with syd-2 (lf) mutants that causes impaired locomotion (Liao et al. 2004; Noma et al. 2014).
The continued assembly of the NPC and NE throughout the yeast cell cycle has been used as a basis for genetic screens, selecting for mutants that caused mislocalization of tagged Nups.
If so, it would be interesting (in future studies) to examine mutants that caused the SPB to be positioned further away from the cell cortex to see if this changed the timing of mitotic entry.
Importantly, 2D-gels results on replication bubbles were further and fully confirmed in analysis of origins by BrdU incorporation and hybridization of BrdU-labeled DNA on Affymetrix tiling arrays on mrc1 and rad53-11 mutasts, as well as on the mec1-1 muthat that cause fork stalling and early activation of late origins, respectively [40], see Figure S5.
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