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Indeed, none of the S. cerevisiae mutants tested was protected from the cytostatic/cytotoxic potential of all these compounds.

Under the oxygen-limiting conditions used in these fermentations, which resemble the typical anaerobic conditions found in large-scale VHG fermentations, the profile of CO2 production obtained in wild-type cells and in the different mutants tested was similar to the profile of ethanol formation, which indicates that most of the CO2 produced came from the fermentative pathway (results not shown).

The differences observed in tropistic responses were not due to growth limitations since the growth rates among all the mutants tested were not significantly different from that of the WT.

Remarkably, all mutants tested were able to pulldown GFP-Sox2 (Figure 5A).

Again, as the two mutants tested are maintained in different genetic backgrounds, the wild type mice display somewhat different initial preferences for the flavored food (Figure 5).

To our surprise, albeit to somewhat reduced levels, all of the 11 50 aa scanning deletion mutants tested were able to rescue tsBN67 cell proliferation at the nonpermissive temperature.

Similarly, the sensitivity of deamino-NADH oxidase activity to capsaicin of the thirteen mutants tested were all similar to the wild type.

Among the mutants tested were a type III secretion mutant (pscD), mutants in three different type III virulence effectors (ExoT, ExoU, and ExoY), an exoTUY triple mutant, a mutant in a secreted exotoxin (toxA), and two mutants in a gene involved in the production of toxic hydrogen cyanide (hcnC).

The mutants tested were those relevant to our evolutionary model, including a deletion of N-terminal insertion (αHext−/βA mutant, where amino acids 6 27 have been deleted), a deletion of the proline-rich wrist region insertion (αHPWR−/βA mutant, where amino acids 68 85 have been deleted) or in tandem deletion (αHext-PWR−/βA mutant) (Fig. S4, Table S3).

The list of alleles of JmjC mutants tested is presented in supplementary Table S1 online.

However, the other three premature stop seipin mutants tested were robustly expressed.