The latter approach has paid substantial dividends with the drug ivacaftor for CFTR gating mutants such as G551D.
The molecular principle behind drug resistance of the protease mutants such as (V36M, T54S and R155K) is still poorly understood.
This drastic increase in gene-targeting frequency reduced the labor required to isolate auxotrophic mutants, such as an arginine auxotroph, without the use of counter-selective compounds.
Satoh and Omura (1981) induced various endosperm mutants such as 'waxy'dullull'flouryury', and 'sugary' using ethyl methanesulfonate (EMS), ethylene imine (EI), and N-methyl-N-nitrosourea (MNU).
The smg11 also showed similar phenotypes to BR defective mutants, such as reduced leaf angle and small grains (Additional file 1: Figures S5 and S6a).
Single mutants such as F93G or I144G do not disrupt carbohydrate binding and homodimerization capabilities, but abolish the bioactivity of the protein.
Other enzymatic properties of both mutants, such as pH and temperature dependence, were the same as those of the wild-type enzyme.
He observed the filmmakers were "big fans of silent henchmen", due to the small roles of the various villainous mutants; such as Lady Deathstrike.
High-fat mutants such as daf-2 and daf-7 show reduced quiescence on HB101 [36].
Other color mutants such as arp2 and abr1 were at least as virulent as the wild type strain B5233.
The Mtmr8 morphants have morphological traits in common with Hedgehog pathway mutants, such as U-shaped somite boundaries.
