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To investigate this question, we tested for genetic interactions between Not4 and various hnRNPs by overexpressing NOT4 and not4L35A in a variety of hnRNP mutants, some of which have mRNA export defects [19], [20], [21], [22].
We found that, in cye-1 mutants, some of the posterior daughters of the seam cells abnormally adopted syncytial fates in the early larval stages (Figs. 5D and F; data not shown), consistent with the observation that adult cye-1 animals have fewer seam cells than normal [9].
In sy5353 and sy5353 ; bh20 mutants some of the Pn.p appeared small and morphologically similar to P12.pa (Seetharaman et al. 2010).
However, trt-1 ; pot-1 double mutants exhibited delayed senescence in comparison to trt-1 animals, and senescence was further delayed in trt-1 ; pot-2 ; pot-1 triple mutants, some of which survived robustly in the absence of telomerase.
My only suggestion for improvement concerns the initial description of selection in the mutationist framework as "a stochastic filter (a sieve or pruning hook) that modulates the chance of survival of spontaneously arising mutants, some of which get lucky".
The specified structural domain was almost identical for the 3mUB1, 5mUB1, 8mUB1, 3mDB1, 3mUB1_3mDB1 and 5mUB1_5mDB1 mutants, some of which have very different splicing efficiency levels (see Additional file 1).
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One aspect that has become apparent is the elevated number of recombinants among the X4-mutants, some of which produce strains without the X4 mutation.
A similar pattern was evident for all autophagy mutants; although some of these mutants were detected at the reference timepoint and in the pools of proliferating cells, the counts decreased dramatically from 4 wk onwards (Table S2).
The absence of triple mutants and some of the double mutants from the present dataset gives rise to genotopes that are subsets of the cube.
We tested most of the white mutants and some of the blue mutants, including those that had a wild-type response to the CYC1 and SNR13 terminators.
As Leroi says: 'We are all mutants, but some of us are more mutant than others.' And, finally, a golden oldie: 25 years after its first publication, Harvard University Press has rereleased Edward O Wilson's classic work, On Human Nature.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com