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In total, we generated 15 mutants (single to tetra).
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To obtain growth curves of wild-types and resistant mutants, single colonies were used to inoculate at least three biological replicates of MHB starter cultures (2 mL) that were incubated at 37 °C for 24 h shaking at 500 rpm.
We used paired t-test to compare the average colony size of double mutants to single mutants and recorded t-statistics and p-values for each test.
It might be more informative to characterize substrate specificity of these atToc159 isoforms by the plastid proteome from double mutant to single mutant or wild type plants.
To generate fls2 double-1 double mutant, single mutant fls2 was crossed with desi3a-1 single mutant to generate fls2 double-1 double mutant.
We modeled only the single mutants known to confer drug resistance using recently reported dose response data from single-cycle replication studies [ 46].
The vte1 npq1 mutant was crossed with the pdx1 single mutant to generate a triple mutant (vte1 npq1 pdeficientcinnt in vitamins E and B6 and in zeaxanthin.
In addition, esk1-5 aba3-1 double mutants were smaller than both dwarf single mutants due to the combined effects of ABA deficiency and reduced water transport.
Double mutant combinations that were viable behaved in a similar way as single mutants attending to morphology and temperature (not shown).
Cortical lamination in these mutants was analyzed as a read-out of Reelin activity in brain development and compared to single mutants or to double Apoer2/Vldlr mutants, which exhibit a reeler-like phenotype.
However, an enhanced penetrance of big hooks was observed in fsn-1 ; ppm-1 double mutants compared to single mutants.
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