Sentence examples for mutants since the from inspiring English sources

In order to facilitate structural studies of the extracellular domain (ECD) of human α7 nicotinic acetylcholine receptor (nAChR), we designed several mutants, since the wild-type-ECD forms large oligomers and microaggregates, and expressed them in the yeast Pichia pastoris.

The relevant concept here is that of an evolutionarily stable strategy (Maynard Smith and Price 1973; Taylor and Jonker 1978); when a population of individuals adopts such a strategy, it cannot be successfully invaded by isolated mutants, since the mutants will be at a disadvantage with respect to reproductive success.

We first tested the levels of Hcp expression in quorum sensing (QS) regulatory mutants since the QS is the main growth phase mediated regulatory system in V. cholerae.

There was little difference in activation energy for channel opening with the KCNE1 mutants since the kinetics for channel activation were largely unchanged (Table 1).

Notably, we predicted and observed a strong buffering effect for both these mutants since the missergregation rate is by far lower than the product of the CMR for the individual deletions of mad1mad3 and mad2mad3.

These two mutants are very likely dGIPC null mutants since the entire coding region is deleted in both lines based on genomic PCR, and since no dGIPC protein is detected by Western blots in homozygous third instar larval discs, or by immuno-staining in homozygous somatic clones (Fig. 2C,D).

It is also likely that the C-terminal is essential for stability both in the WT nsp4C and C425S mutant, since the expression of a ΔF484-Q496 deletion mutant has very low yield compared to the WT nsp4C protein.

Furthermore, the reduction was not due to a less efficient infection of Ag8 cells by the Delta 40 bp mutant since the viral genomic load, as determined by quantitative PCR, was similar in all samples analyzed (Fig. 7C and D).

The transgenic plants expressing YFP CESA6 were slightly different than those previously published [15] [17] in that we extensively re-screened the T1 generation of the transformation described by Parerdez et al. [14] to isolate a homozygous allele that complemented the procuste (CESA6) mutant since the previous allele was heterozygous.

The KDD7 mutant appeared to be more attenuated than the KDD6 mutant since the time needed to establish chronic infection was longer for the former.

We have not repeated this NMR analysis for the N46Q/V110Q mutant since the measurements of the binding constants provided already clear indications of the success of the design procedure.