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We have generated the three carboxyl-terminus deletion mutants shown in panel D. The wild type and mutant pCDNA3.1-Flag-ARIP3 constructs were co-transfected with pRK7-HA-Tollip cDNA.
We have also quantified the immunoblot signals of the different Gap1 mutants shown in Table 2 and found that none was present at a level less than two-fold compared to the native Gap1 protein (Fig. S3 and data not shown), i.e. their reduced or lack of activity is not due to non-expression.
Six out of seven sites were occupied, as seen from the decrease in mobility caused by the ablation of a consensus site in the single mutants shown in Fig. 2A and B. No change in mobility was observed for the Δ5 mutant expressed either in HEK cells (Fig. 2A) or in the A375 melanoma cells (supplemental Figure S1) suggesting that this sequon is rarely occupied in human tyrosinase.
The sporulation phenotype of rdrp-1Δ and rdrp-2Δ mutants shown in Figure 1A reinforces that involvement.
Images of selected gfp mutants shown in Figure S2 were made using a Leica TCS LSI-III Confocal Microscope System.
All localization mutants (shown in blue) are different from the σF mutant control with p values less than 0.0025 from a paired t-test.
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To gain further insight into the reduced speed Dyrk1A+/− mutants showed in the MWM, we studied the floating behavior of these mice and that of their wild type counterparts.
In addition, previous studies on Δ PpAOC knock-out mutants showed in analogy to studies in flowering plants [ 50, 51], that reduction of cis-OPDA biosynthesis leads to reduced fertility, aberrant sporophyte morphology or interrupted sporogenesis [ 16].
However, elg1 Δ srs2 Δ double mutants show, in comparison with each of the single mutants, increased sensitivity to DNA-damaging agents, such as the alkylating agent methylmethane sulfonate, the inhibitor of deoxynucleotides synthesis hydroxyurea, and the topoisiomerase inhibitor camptothecin.
As an example of Case 2, in the mutant shown in Code 4 (Additional file 1: Figure S4), w 2 is associated with both original (Code 3 - Additional file 1: Figure S3) and mutated files.
Interestingly, as opposed to the dramatic attenuation of the Y. pestis nlpD mutant shown in the present study, the Y. enterocolitica nlpD mutant displayed only a limited impairment of virulence [45].
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