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An approach based on the site directed mutagenesis was followed, with the preparation of two mutants showing a total loss of activity.
Our results might be of help for the rational design of enzyme mutants showing a biotechnologically relevant substrate specificity, particularly to be used in bioremediation.
Among the assayed clones, 76 mutants showing a six- to sevenfold higher activity than the wild-type enzyme were selected and further screened in flasks, leading to the selection of the best five variants exhibiting up to two- to threefold higher activity compared to the wild-type enzyme (Table 1).
Thus, despite both pbl1 and bik1 mutants showing a reduced MAMP/DAMP-induced calcium elevation, downstream growth arrest effects differ.
The biological significance of transcriptome modulation seen here is confirmed by the DNA repair mutants showing a mild sensitivity to oxidizing agents.
The Chl/Car ratio differs significantly between the two genotypes, with wild-type plants showing a 24% reduction under stress, and mutants showing a 38%reductionn.
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Rice plastochron (pla) mutants show a short plastochron and small precocious leaves.
Four mutants show a higher reaction rate with the radical compared to the wild type.
Furthermore, all mutants showed a significant 40 °C increase in optimum temperature compared to wild-type enzyme.
The rice mutants lacking AS1 (as1 mutants) showed a decrease in asparagine content in the basal portions of shoots.
Rice mutants lacking GS1 2 (gs1;2 mutants) showed a remarkable reduction in the contents of both glutamine and asparagine in the basal portions of shoots.
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