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Among the candidate mutants, the A8T, A92E, N97Q and T245S mutants showed higher organic solvent stability at various methanol concentrations.

With the addition of 3% MnCl2, all the mutants showed higher ethanol production from 14.11 to 15.72 g/L compared to the R-control which produced 9.8 g/L, and the other used mutants with 1, 10 and 20% concentrations of MnCl2.

The SCCmec-deleted mutants showed higher colony spreading than their respective parent strains (Figure 1C and 1D).

DopRf02676 mutants showed higher initial levels of activity (8.6 mm/sec), and while DopRf02676 flies also acclimated, they maintained two-fold higher activity levels (4.9 5.3 mm/sec) (Fig. S6B).

The Δ ppsA and Δ ptcE mutants showed higher trehalose levels under all conditions.

By contrast, the quadruple- DELLA mutants showed higher expression of AOX1A and RDR1 than the non-mutant Ler plants.

The rpn8a-2 and rpt5a-4 mutantshoweded high sensitivities when exposed to high-B conditions (1.5 6 mM) after the embryonic stage, suggesting that RPN8a and RPT5a are involved in high-B tolerance starting at least at post-embryonic stages (Fig. 2e, f and Supplementary Fig. 5).

While wild-type mice displayed the expected low amplitude (Type I) phase response curve (PRC), Rev-Erbα−/−Per1Brdm1 double mutants showed high amplitude (Type 0-like) resetting (Fig. 3A D) [24], [24].

As observed through flow cytometry, none of the mutants displayed ability of ROS reduction; contrarily, cells expressing Magmas mutants showed high level of ROS as compared with control.

Group I had lower levels of antheraxanthin and zeaxanthin at LL than wild-type and most of these mutants showed high O2 production and increased GPXH expression.

Afh mutants show higher timing uncertainty compared to control mice.