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Measurement of telomere repeat fragments (TRF) in the breast cancer cell lines stably expression TGF-β family cytokine receptor mutants showed consistent results.
Field experiments comparing wild type (WT), single (gpc-A1 and gpc-B2), and double (gpc-A1/gpc-B2) mutantshoweded consistent results across the four tested environments UCD-20122, TAU-2012, NY-2012 and NY-2013, Figure 2, Additional file 1: Figure S1 and S2).
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The two independently derived mutants always showed consistent sensitivity to the drugs tested.
Genes that were down-regulated in the microarray experiment and potentially involved in different aspects of the ashh2 mutant phenotype showed consistent expression changes by real-time PCR (Figure 6C, Table 3).
By using a 3-fold cutoff, the hrde-1 mutant animals showed consistent increases in Pol II occupancy at 191 1-kb regions in all three sets of Pol II ChIP samples (8WG16, S2, and S5).
∆rpoS is the only tested mutants showed a consistent higher log reduction than that of the parental strain at all RH conditions.
Mutant (Fig. 1b) and morphant (Fig. 1c) zebrafish, reared to 120 hour post fertilization showed consistent phenotypes.
Only Arsenal have showed consistent form.
The F1 progeny of a cross between the as1-21 and as1-20 mutants showed abscission defects, consistent with an allelic relationship between the mutations.
Wild type strains exhibited weak amplification of the extended cDNA, while tfc6 mutants and B-box mutants showed stronger signals, consistent with the presence of 5′-UTR extensions in strains where adjacent Pol III complex assembly was either globally or site-specifically impaired.
Indeed, when we performed in situ hybridization for Pdgfra in Wnt1-Cre; Pdgfrafl/fl mutants and Fgfr1 expression in Wnt1-Cre; Fgfr1fl/fl mutants, we found some receptor mRNA expression in the E13.5 palate of the respective conditional mutants (data not shown), consistent with our qPCR results.
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