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These results indicate that both the N-terminal and helicase domain of dRecQ4 are indispensable for animal viability, although the N-terminal domain alone is sufficient to rescue the mutants' sensitivity to gamma irradiation.
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To further establish that Set2 functions in cellular survival against genotoxic insults, we selected 30 genes representative of the pathways involved in DDR and repair and analysed double mutant sensitivity to MMS.
The role of ROM2 in microtubule organization is further indicated by S. cerevisiae rom2 mutant sensitivity to the microtubule depolymerizing drug benomyl [25].
In fact, Elp3 appears to be antagonistic to Mst2, because Δ elp3 suppresses Δ mst2 mutant sensitivity to high salt concentrations and HU.
The specificity of cysl-2 mutant sensitivity to cyanide suggests that this enzyme is directly acting to diminish HCN levels rather than to affect H2S levels.
The Arabidopsis chloroplastic GRXs, AtGRXcp and AtGRX4, were induced by various stresses and suppressed the yeast grx5 mutant sensitivity to oxidants.
The genetic screen for mutant sensitivity to Octodrine was tested with three C. albicans libraries that were previously created in [ 15– 17].
When the responses to ABA and salt stress were studied in these mutants, only the cyp709b3 mutant showed sensitivity to ABA and salt during germination.
Since mutation of SSN8 caused abnormal morphology, we further examined if the ssn8 mutants showed sensitivity to stress reagents.
Deletion mutants showing sensitivity to at least one reagent were picked to create a sub-library.
These 28 mutants showed sensitivity to treatment with neomycin and/or streptomycin.
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