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To test whether these ESRTs also contribute to cellulase secretion, we next checked the cellulase production of 18 mutants sensitive to 3 mM DTT and 31 mutants with sensitivity to 5 mM DTT in liquid batch culture.
Microarray-based screens were used for en masse identification of individual mutants sensitive to methyl methanesulfonate (MMS).
To define factors in E. coli promoting survival to replication fork stress, we isolated insertion mutants sensitive to replication inhibitors.
Here, to identify the landscape of genes and cell functions involved in cadmium resistance, we have screened the Saccharomyces cerevisiae deletion collection for mutants sensitive to cadmium exposure.
In conclusion, our results provide a rationale for further clinical evaluation of darolutamide in enzalutamide-resistant CRPC, in particular in combination with circulating tumor DNA assays that detect AR mutants sensitive to darolutamide, in a precision oncology setting.
The overlapping sensitivity of our diploid DOX sensitive deletion strains to the G1 specific toxin zymocin as well as with mutants sensitive to oxidative damage and those that regulate cell size control in G1 suggests that a significant fraction of the lethal activity of DOX occurs in the G1 phase of the cell cycle.
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Both cac1 (the yeast CAF1 gene) and asf1 mutants are sensitive to UV, but a cac1 asf1 double mutant is more sensitive than either single mutant [ 120, 121].
In total, 53 of the 73 EcoRIs non-RAD52 group mutants were sensitive to bleomycin (4 μg/ml) and 44 mutants were sensitive to MMS (2 mM).
To determine whether ND2 mutants were sensitive to hyperoxic conditions, we housed young ND2 mutants and controls in 100% oxygen and monitored their lifespan.
Surprisingly, the behaviours of ΔrecG ΔclpX and Δruv ΔclpX mutants were very different from each other despite both mutants being sensitive to 2-AP.
Seven null mutants remained sensitive to GEL, whereas the growth of the ΔnemA mutant was partially recovered at 30°.
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