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This plasmid was electroporated into M. smegmatis mc155 and mutants selected in a two-step mutagenesis process by single cross-over mutant selection on Luria agar containing hygromycin (50 µg/mL), followed by selection of katG deletion mutants on sucrose-containing agar.
About 30 mutants selected in bacteria were then tested in HeLa cells, transfected with plasmids obtained after cloning of FP genes into pcDNA3.1 plasmid (Invitrogen/Thermo Fisher Scientific).
We therefore investigated whether the capsid mutants selected in vitro can target PymT breast tumors in vivo.
This high degree of conservation was further confirmed by experiments showing that E4orf4 mutants selected in yeast for loss of their ability to induce toxicity, were also deficient in their ability to induce cell death in mammalian cells [23].
In addition to the fluoroquinolone resistant mutants selected in the presence of ciprofloxacin, strain 50 64 was further examined to evaluate if a total or partial reversion of the resistance phenotype could occur under non-selective conditions.
Bacterial mutants selected in this way are used to re-infect macrophages.
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c, Discharge activity towards Tris and glycerol for all of the tested MYCBP2(cat) cysteine-to-serine mutants (selected mutants shown in Fig. 2c).
Aneuploidy was strikingly emergent in C. albicans mutants selected for mouse colonization in experiments relayed by Norman Pavelka (Singapore Immunology Network).
This characterization of T-DNA insertions in mutants selected without regard to phenotype supports application of Agrobacterium-mediated transformation as an insertional mutagen for genome-based screens and functional discovery of genes in Histoplasma.
We agree that these bacteria might represent preexisting mutants selected during drug exposure in a certain drug concentration window.
Here, we present a microarray-based approach to identify deleted genomic regions in rice mutants selected from a large collection generated by gamma ray or fast neutron treatment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com