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However, a model in flies whereby ethanol-evoked release of dopamine in DAT mutants results in higher-than-normal extracellular DA levels does not fit in a simple manner with the reduced hyperactivity of DAT mutants seen in our experiments.
This correlates well to the proposal that p110β has naturally acquired the oncogenic mutants seen in p110α (Brown and Auger, 2011).
However, the extremely severe phenotype of CSP mutants seen in Drosophila [ 60] is not observed in C. elegans null mutants [ 61], so even if mutation of a worm PAT s) completely prevented CSP palmitoylation, this would not result in strong effects on the phenotypes analysed in the present study.
An enhanced effect of the Ku70 mutants seen in the two-hybrid compared to the PCA are explained by the inherent irreversibility of the YFP fragments folding in the PCA, which we reason lessens the degree of disruption of the Ku70-TRF2 interacausedcaused by the mutations.
These revertant colonies are reminiscent of the mixed colonies with lac amplification mutants seen in the Cairns system (Andersson et al. 1998; Slechta et al. 2003; Kugelberg et al. 2006), which will be described elsewhere (S. Quiñones -Soto and J. R. Roth, unpublished results).
Similar(55)
Surprisingly, decreased transcription of genes important for hemolytic activity, i.e. FruB and the transcriptional anti-terminator SACOL0403 (Table 3), were observed in the Δstk1 mutant (see # in Table S2).
In the L1 stage, we observed low levels of perinuclear P granules in the PGCs of meg-1 meg-3 meg-4 triple mutants as seen in meg-3 meg-4 double mutants.
The levels of ceh-2 :: gfp and daf-6 :: yfp were frequently below the detectable limit, whereas cdh-3 :: gfp was often reduced in the mutants (see vulF in Figure 2, L and L′) or missing (not shown).
Six of 14 kinase-unknown mutants were seen in codon 469.
About 1 log kill of the mutant was seen in both activated and resting macrophage at 6 days of infection.
This 19% reduction in the Kcat correlates well with the minor loss of structure for this mutant as seen in Figure 3, which is also 18%.
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