As mentioned earlier, data in transiently transfected mutant COS-7 cell lines using an ELISA-based read-out with mGlu1 antibodies revealed that the majority of mutants retained plasma membrane expression comparable to WT mGlu1.
Like ndc80-21, these loop-less mutants retained kinetochore-localizing activities.
The E204A and Q242A mutants retained 85%and83%3% activity, respectively and the W244A mutant retained full activity compared to the wild-type (Table S4), suggesting lesser roles for these residues.
The mutants retained their high resistance to pepsin.
Four Hhat mutants with deletions within predicted loop regions and five point mutants retained stability but lost palmitoylation activity.
Most of the H5 HA mutants retained affinity for the 3SLN glycopolymer.
These PP1-binding deficient mutants retained interactions with endogenous actin (Fig. 7B).
The second group of mutants retained partial (20 50% of WT) activity (G217A, F338A, W378A, H379A).
All these mutants retained infectivity.
Importantly, the GOF mutants retained potassium selectivity (Supplementary Figure S1B).
