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One possibility is that the increase in TUNEL staining observed in these various mutants results from defects in the kinetics and progression of PCD, a phenotype sometimes observed in apoptosis deficient strains (e.g., see [10], [22]).
grp (Chk1 mutants) results from a p-element insertion and is a genetic null (Fogarty et al., 1997).
Lenssen et al. (2005) have shown that the constitutively high level of STRE-driven expression in ccr4 -not mutants results from two independent effects.
A phenotype identical to that of whiA mutants results from mutations in whiB (SCO3034), which encodes one of the actinobacterial signature proteins (Table 1A, Fig. 3).
Thus, we propose that the defective cellular organization in Celsr1 Crsh and Vangl2 Lp mutants results from disruption to Rho kinase function which likely leads to cytoskeletal defects, thus perturbing tissue structure.
We then tested whether the expansion of the ZLI in Pax6 −/− mutants results from the misspecification of cells flanking the normal position of the ZLI (e.g., rather than an overproliferation of correctly specified ZLI progenitors).
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While some local residents joked that the deer were mutants resulting from nuclear weapons materials stored on the grounds, and others mistakenly thought they were albinos, a few, like the environmental photographer Leland Brun, recognized that they were a rare offshoot of the white-tailed species.
This also facilitates detection of deletion mutants resulting from errors during primer synthesis.
Most spontaneous Pgm– mutants result from the deletion of a 102-kb chromosomal region termed the pgm locus [5], [6].
All are apparent null mutants resulting from Agrobacterium tumefaciens-mediated, multi-kb insertions that disrupt the genes [33].
Loss-of-function of these mutants resulted from altered channel kinetics, including a negative shift of steady-state inactivation and a reduced voltage dependency of open-state inactivation.
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