Furthermore, overexpression of TET1 mutants resulted in a stronger inhibitory effect on neuronal differentiation even than that of overexpression of WT TET1 (TET1) (Fig. 5E).
Also, Xiao et al. showed that depleting SDH in mice or ectopic expression of tumor-derived SDH mutants resulted in the accumulation of succinate [83].
The impaired leucine feedback system of these mutants resulted in increased 3H4MV fraction due to the increased metabolic flux to leucine biosynthesis.
Three thermostable single mutants (F48Y, T50V, and T147L) were experimentally identified, and combination of the single mutants resulted in a more thermostable triple mutant (F48Y/T50V/T147L).
Analysis of the flanking sequences of these insertional mutants resulted in accumulation of nearly 448,000 gene-tagging sequence resources for characterization of gene functions (Wei et al. 2013).
Our SEM and TEM observation results showed that, at the mature pollen stage, the granular and collapsed bacula in deformed exine of oslap6 mutants resulted in sterile and aborted pollen (Fig. 3).
Combination of the positive mutants resulted in the best mutant M31 that decreased its pH optimum for 1.5 units and showed increased catalytic activity at pH < 5.0 compared to the wild-type enzyme.
Our detailed analysis of the ΔC3GMS mutants resulted in several important findings.
Basal or overexpression of phospho-mimicking or putative non-phosphorylated Sso1p or Sso2p mutants resulted in no obvious growth phenotype.
Within the α helical segment two alanine mutants resulted in enhanced rates of deactivation (I19A and R20A).
An increase in phyB level, relative to Col WT, in the single and double pif mutants resulted in a strongly inhibited hypocotyl (see Figure 8 and Table S1).
