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Most of the mutants result in alteration of catalytic specificity, yielding both galactooligotetrasaccharide (GOS4) and galactooligotrisaccharide (GOS3) compared to the sole product GOS3 by the WT.
Previous studies showed that the ssIIIa mutants result in a chalky interior appearance in kernels and an irregular shape of starch granules caused by insufficient crystallization of starch (Fujita et al. 2007; Ryoo et al. 2007).
Measurements of respiratory activity in rSgAOX expressed in Escherichia coli FN102 membranes indicate that all mutants result in a decrease in maximum activity of AOX and in some cases (D163 and L177) a decrease in the apparent Km (O2).
In conclusion, the expression of the R137C and R137L mutants result in a background production of cAMP leading to a constitutive water reabsoption.
One explanation might be that some premature stop codons in the dpolv mutants result in nonsense mediated decay of the mRNA [36] and this completely removes all functions in the olv class.
All mutants result in practically identical phenotypes, as assayed by histology, immunohistochemistry and in situ hybridization, suggesting that the C-terminus of Sec24D, the common deletion in all mutants, is critical for protein folding and dimerization.
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Three thermostable single mutants (F48Y, T50V, and T147L) were experimentally identified, and combination of the single mutants resulted in a more thermostable triple mutant (F48Y/T50V/T147L).
Thus, it is possible that the duration of cell proliferation in developing leaves is insufficient in pla/slr1 double mutants, resulting in suppression of the slr1 phenotype.
Also, Xiao et al. showed that depleting SDH in mice or ectopic expression of tumor-derived SDH mutants resulted in the accumulation of succinate [83].
Analysis of the flanking sequences of these insertional mutants resulted in accumulation of nearly 448,000 gene-tagging sequence resources for characterization of gene functions (Wei et al. 2013).
Furthermore, overexpression of TET1 mutants resulted in a stronger inhibitory effect on neuronal differentiation even than that of overexpression of WT TET1 (TET1) (Fig. 5E).
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