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Second, the fact that mutants responsive to CAI-1 showed an increase in bioluminescence, points to a possible mechanism for understanding the effects of cell-free culture fluids from the fast-growing Vp strain on Vh.
By reverse engineering, Roth and colleagues were able to introduce analogous mutations into the other members of the muscarinic receptor family and generate RASSL mutants responsive to CNO for the M1-M5 muscareceptorseptors [ 36 ].
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For the two different AI receptor mutants (each responsive to a single autoinducer) that they studied, they observed a coefficient of variation cv ∼0.2 0.4 in the gfp expression, significantly smaller than the cv ∼1 that we observe here in V.fischeri luminescence.
Overall, our rps19 model, as well as rpl11 mutants is responsive to L-leucine treatment.
We verified by quantitative reverse transcription PCR (qRT PCR) that gcn-2 mutants were responsive to feeding RNAi at the same level as N2 (Fig. S5).
In order to determine whether spt mutants are responsive to reduced levels of GA, we exposed seedlings to the GA biosynthesis inhibitor paclobutrozol (PAC) and examined the effect on meristem size.
All three mutants were responsive to SAG, showing that they were not disabled, but demonstrated substantially reduced 20(S -OHC binding and reS -OHCveness, with the mSmo Y134F bindingompletely unresponsive.
These data show that the K624R mutant remains responsive to both GSMs and the iGSM showing relatively unchanged Aβ profiles and MIs relative to those of the WT.
These two mutants appear to generate a novel phenotype of dnaQ mutants hyper-responsive to nalidixic acid induced cleavage complexes (see Further discussion).
PDGFRA mutants were not responsive to their ligand, PDGFAA, and displayed a high constitutive kinase activity.
Basal secretion of serum corticosterone was not distinguished by genotype; however, corticosterone levels in mutants were more responsive to stress.
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