These are comprised of data on the numbers and resistance properties of mutants resistant to each of the drugs.
For mutants resistant to ciprofloxacin (Supplementary Fig. 6a) and trimethoprim (Supplementary Fig. 6j), resistance mechanism was non-significant, but it was a significant factor for those resistant to mecillinam (Supplementary Fig. 6d) and nitrofurantoin (Supplementary Fig. 6g).
The existing PR mutants resistant to the competitive PIs are less likely to confer cross-resistance to the allosteric PIs because allosteric inhibitors do not compete with substrates for the active site.
We determine collateral susceptibilities of mutants resistant to relevant antimicrobials against 16 antibiotics.
In general, mutants resistant to ciprofloxacin and mecillinam displayed severely reduced growth rates, suggesting high costs of resistance.
Mutants resistant to mecillinam required only a single selection step, while multiple selection steps were required to select for resistance above clinical breakpoints for the remaining antimicrobials.
Mutants resistant to trimethoprim contained mutations either in folA and/or its promoter or genetic amplification of a large region containing folA (Supplementary Data 4).
In mutants resistant to ciprofloxacin we also observed conserved CR towards chloramphenicol, as reported in ref. 21, but not in ref. 23.
SNPs present in the wild-type assembly or in at least two mutants resistant to different antimicrobials from the same strain background were excluded.
Roncero, C., Valdivieso, M. H., Ribas, J. C. & Duran, A. Isolation and characterization of Saccharomyces cerevisiae mutants resistant to Calcofluor white.
Mutants resistant to nitrofurantoin and trimethoprim displayed lower fitness effects, and several resistant mutants harbored apparent cost-free resistance mutations (Supplementary Fig. 4).
