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The Δ5G-ver1, Δ5G-ver2 and Δ3G were also constructed by site-directed mutagenesis from the series of deglycosylated mutants reported previously [2].
As such, ProcM mutants are more general as leader peptide dependent kinases than LctM mutants reported previously, and they may find utility as enzymatic tools for phosphopeptide synthesis.
ILK mutant embryos present an enlarged common arterial trunk similar to that of PINCH1 mutants, but strikingly different from that of other mutants reported previously, suggesting a unique role of ILK-PINCH1 in cardiac NCC and a previously unappreciated role for cardiac NCC in OFT formation.
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The R378G mutant reported previously in an Iranian PARK15 family [11] was also tested.
High stringency datasets for the Nrl mutant were reported previously [25], and low stringency datasets for the Nrl mutant were obtained as described above.
However, while all four of the downregulated deletion mutants functionally impacted CLS, only leu3∆ and add66∆ were long-lived compared to WT. Extended CLS of the leu3∆ mutant was reported previously [ 26, 27].
Isolation and properties of RSV tsNY68 mutant were reported previously (Kawai and Hanafusa, 1971).
Confocal analysis and quantification of at least 200 transfected cells showed a predominant cytoplasmic localization of the PTENK289A mutant, as reported previously.
This result, together with the mutant phenotypes reported previously, supported the idea that HEC1 and SPT might functionally interact throughout female reproductive development.
Results of a genetic cross where only one of the parents was deleted for both donor loci, mat1-P (donor-deleted) X mat2-P − donor locus mutant, was reported previously (Furuya and Niki 2009): among 78 octads analyzed, two had changed mat1-P to mat1-M in donor-deleted segregants.
The kinase activity of the single mutants has been reported previously by various groups.
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