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By contrast, expressing UNC-13L in unc-13 s69 unc-13 s69econstituted only fast release.
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The phenotype of created mutants was compared to the wild-type P. temperata and to mutants reconstituted with the respective complementary plasmid (complementation of the mutant with the respective intact gene).
Thus, restoring UNC-13L expression in unc-13 [L−S+] mutants reconstituted both fast and slow release.
By contrast, UNC-13L expression in unc-13 s69 unc-13 s69utes only the EGTA-reconstitutesponlythef release.
To access the agar invasion phenotype, the wild-type, ssn8 mutant, reconstituted, and overexpression strains were incubated on YPD medium (Materials and Methods S1).
(B ) Activity of purified wildtype MjNhaP1 (WT) and the N160D mutant reconstituted into proteoliposomes under symmetrical pH.
A combination of GPSLAT-2 and ΔTM2-7 reachtors, each deficient in rescuing developmental defects of lat-1 mutants, reconstitutes full receptor function.
Mutant E113C/Basal mutant only formed a pigment (λmax = 380 nm).
The mutants were reconstituted by transforming a reconstituted construct for each gene.
Deletion mutants and reconstituted strains were then created from strain R265 according to an established protocol [71].
These NTSR1 mutants were reconstituted into 75%POPC/255% POPG nanodiscs and then activated by agonist binding for phosphorylation assays.
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