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Many kinetochore mutants provoke a strong checkpoint arrest (e.g., cse4-1, scmif2-3mif2-3, ame1-4, okp1-5, nnf1-17, mtw1-1, stu2-277, dam1-1) but a subset does not (e.g., ndc10-1, cep3-1, spc25-7, ndespite deseveresevere defects in chromosome-MT binding.
Analyses of epithelial and mesenchymal markers by both Western blot (Fig. 5A) and immunofluorescence (Fig. 5B) showed that stable expression of wild type or LOXL2 mutants provoke a complete loss of E-cadherin and ZO-1 with the concomitant increase in mesenchymal markers (N-cadherin, vimentin and fibronectin) and the F-actin cytoskeleton exhibiting a typical organization of mesenchymal cells.
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Consequently, use of sulfonamides is widespread in Paris and Lyon, favoring the emergence of mutants and provoking a high risk for incidental acquisition of these mutants, even in patients not directly exposed to sulfonamides.
We have reported before that fga loss-of-function mutations leads to accumulation of Sima protein in normoxia, and constitutive expression of a hypoxia-inducible reporter in transgenic lines; abnormal accumulation of Sima in these mutants provokes lethality [20].
Altogether, these results suggested that overexpression of Pkd1l2, which was demonstrated at the protein level in both mutants, provokes neuromuscular disease in mice.
We therefore hypothesize that the inefficient buffering and shaping of the Ca2+ waves operated by mutant mitochondria provokes a local increase in [Ca2+]c and that this, in chronic conditions like as in AFG3L2-associated diseases, triggers PCs degeneration.
To investigate whether the differences in ethylene production between acs2-1acs6-1 acs2-1acs6-1 acs2-1acs6-1nts were sufficient to provoke a differential ethylene response, expression of primutantshylene responsive genes wandmeasured in both genotypes.
Uniforms provoke a certain feeling.
One line, though, might provoke a titter.
It may provoke a new intifada.
But the vote could provoke a bloodbath.
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