The L266F mutant enzyme produced much more 2-hydroxybiphenyl (2.43 vs. 0.1 μg/L) and 3-hydroxybiphenyl (1.97 vs. 0.03 μg/L) than the wild-type.
Figure 5 shows that mutant Mut-4 produced much less bikaverin when the basal medium had inorganic nitrogen sources or soluble, digested protein sources like extracts and peptone.
A team led by Richard Williamson of the Australian National University in Canberra noticed that a mutant Arabidopsis variety produced much less cellulose when grown in soil that was hotter than normal--31 degrees Celsius instead of 18.
This concept was supported by three independent lines of evidence including excess CPAP induced longer cilia (Fig. 3B,D, lower panel), while depletion of CPAP (Fig. 2D,H, lower panel) or overexpression of CPAP-377EE mutant (Fig. 3B,D, lower panel) produced much shorter cilia.
Again, U87dn mutant cells defective in both IRE1 kinase and IRE1 RNase activities produced much lower amounts of EREG under basal condition, a partial recovery of EREG transcript accumulation being observed after 4 to 8 h of incubation with tunicamycin.
Neither action has produced much.
The I707L mutant KlenTaq1 has a higher 74°/37° activity ratio between 4 and 7 and produces much higher yields of difficult-to-amplify target PCR products.
When IFS was introduced into an A. thaliana tt6/ tt3 (transparent testa) double mutant, which has structural defects in both flavanone 3-hydroxylase (F3H) and dihydroflavonol reductase (DFR), and produces much lower concentrations of flavonoids and anthocyanins, genistein levels were much higher than those produced in the non-mutant transformed plants (Liu et al., 2002).
IT DOES not produce much.
However, some retirees produce much more than we might expect.
