Of the more than 500 temperature sensitive mutants isolated in early screens, we focused on mutants that were characterized as embryonic lethal or reported to have eggshell defects (osmotic sensitivity, irregular shape, and unshelled or thinly shelled embryos).
Hence, ProcM is able to perform catalysis not only on substrates with dramatically varied core sequences but also on mutants that are significantly truncated in the leader peptide.
Wildtype Dictyostelium amoebae feed on bacteria, but for decades laboratory work has relied on axenic mutants that can also grow on liquid media.
In vitro assays of the KDAC activity of these two mutants on RutR demonstrated that their KDAC activities were completely abolished.
Previous infection studies with TRI5 knockout mutants on wheat revealed that the trichothecene deficient mutant is still able to infect the inoculated wheat spikelet like wild type [ 9, 11- 13].
The mutants colonies that appeared on the LB plates containing 50 μg/mL kanamycin were subsequently cultured in LB broth and used in HCT-8 cell interaction assays according to the method described above.
When I started working on deaf mutants, people that had had mouse lines in their laboratories for many years were very happy to send them to me with no strings attached.
Both the Knicks and the Nets are mutants — teams that keep evolving, on their way to someplace else.
We found that formation of the Noc4p-Nop14p subcomplex and association with pre-ribosomes were always coupled in all the mutants analysed but that on the other hand Nop14p is not strictly required for the incorporation of Noc4p into pre-ribosomes.
In other words, it is expected that steric hindrance is not significant on the mutants that are labeled well.
