However, designed mutants of this enzyme capable of degrading organophosphates have to be engineered to enhance their thermostability.
Moreover, data obtained with deletant mutants of this family of proteins indicates that they have potential as targets for novel antifungals.
Here we show that analysing the unfolding thermodynamics of single-point mutants of this chimeric fusion protein constitutes a very useful approach to deciphering the thermodynamics of SH3 ligand interactions.
This apparent global suppressor effect suggests that increasing the thermodynamic stability of p16 can be used as a general strategy to restore the biological activity to defective mutants of this important tumor suppressor protein.
The inhibition by charybdotoxin of A-type potassium channels expressed in Xenopus oocytes was studied for several splicing variants of the Drosophila Shaker gene and for several site-directed mutants of this channel.
Since the N-terminus has been previously shown to be important for the degradation of damaged D1, the possibility that the highly conserved cysteine 18 residue situated in the N-terminal domain of D1 is involved in the targeted repair process was tested by examining site directed mutants of this and the other cysteines of the D1 protein.
This represents the first study in which mutants of this type have been investigated for their ability to influence metabolism.
T-DNA insertion mutants of this gene were obtained from the INRA and Salk collections (INRA Flag 240B06; Salk 024194).
However, we have not detected a significant alteration of tolerance to caffeine in any of the mutants of this pathway that we have tested (Supplementary Figure S4).
Mutants of this strain unable to produce microcystin were obtained by insertion of a chloramphenicol (Cm -resistance Cm -resistancehe genes mcartridgecyH, respectinely [30], [31].
Because eEF1A delivers aa-tRNA to the ribosome and cognate codon:anticodon interactions stimulate its GTPase activity, mutants of this factor could alter translational fidelity in two ways.
