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(i) Open probabilities for the wild-type and mutants of single SaTRIC channel at +20 mV holding potentials (same condition as in e).
(h) Current amplitude for the wild-type and mutants of single SaTRIC channel at +20 mV holding potentials, n=4 for each group.
While dominant gain of function mutants show severe dwarfism [ 18, 19], knock out mutants of single or even several ASKs display no or only very slight phenotypes [ 21].
The dataset consisted of the phenotypic outcomes from a set of knockout mutants of single genes and was used to define the classes "essential" and "non-essential" for our reactions.
To further verify whether the α- l-arabinofuranosidase and endo-xylanase activities of Ac-Abf51A were derived from the same module and the active center, truncation mutants of single module (Ac-Abf51An and Ac-Abf51Ac) and substitution mutants (Glu175 and Glu294) were performed using the Quik-Change method (Stratagene).
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Single or various double-mutant combinations of single mutant alleles showed either no or only small defects in leaf growth.
Among all single mutants of the single-repeat R3 MYB genes, only the try mutant displays significant trichome clusters on leaves and shoots [ 1, 2].
Castellarnau et al. estimated that cell wall and membrane conductivities vary up to 70% for isogenic mutants of a single strain of E. coli.
Increased actin bundling has also been observed in knockout mutants of the single AIP1 gene in the moss Physcomitrella patens (Augustine et al., 2011) and in hypomorphic flr mutant alleles in Drosophila (Ren et al., 2007).
The system was able to detect single-plex and multiplex PCR amplicons from various infectious agents as well as wild type and mutant alleles of single nucleotide polymorphisms.
No other single mutant of the single-repeat R3 MYB gene family displayed ectopic trichome formation on the inflorescence stems or pedicels.
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