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Similar phenotypes have been observed in mutants of other negative regulators of the EGFR pathway such as gap1, argos and sprouty [12], [16]-[18].
We demonstrated that the infection of human bronchial epithelial cells and macrophages caused the profound release of type I IFNs and other pro-inflammatory cytokines analogous to ΔNS1 mutants of other subtypes [45].
Replication-defective mutants of other large DNA viruses, including herpes simplex virus [34], [35], [36], [37], adenovirus [38], and vaccinia virus [39], have been produced for use as vaccine vectors, but this approach has not yet been feasible with MVA.
This postnatal decline is a robust process, not affected in null mutants of other myosin isoforms [20] or in animals where development of adult isoforms is retarded by undernutrition [36].
Mutants of other subunits of EMC were not available.
We further examined stage 14 spi embryos and stage 14 mutants of other Egfr pathway components.
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The growth experiment included wild-type plant, two independent mutant lines of fpgs1 (fpgs1 - 1 and fpgs1 - 3 ), knockout mutant of other two isoforms of FPGS genes (fpgs2 -mitochondiral and fpgs3 -cytoplasmic) and complemented transgenic lines of fpgs1 - 1 [ FPGS1c (pFPGS1::FPGS1 - GFP :: fpgs1 - 1 )].
Mutants of the other subgroup (IA), on the other hand, had strongly reduced α-tocopherol levels, especially in HL grown cultures, and either lowered or not changed NPQ compared to the wild-type strain.
Although best known in the context of loss-of-function mutants, combinations of other types of perturbations can also result in synthetic lethality, including overexpression of genes, the action of a chemical compound or environmental change [5 8].
The comparison of the gene expression profiles of our DnaJ mutants with those of other mutants deposited in the public microarray database further supported the idea that the tolerance of the DnaJ mutants to oxidative stress induced by MV is due to the trigger of a global stress response in these mutants (Additional file 6 and 7).
To test whether HRDE-1-dependent silencing events also require other nuclear RNAi pathway genes, we performed mRNA-seq analysis for mutants of three other core nuclear RNAi factors [ nrde-2 gg91), nrde-2 gg91) anrde-3 gg66g129)]. nrde-3 gg66
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