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Further, we screened ten BY4741 single-deletion mutants of genes involved in the competing pathways for 2-PE production, and found that strains aro8Δ and aat2Δ displayed increased 2-PE production.
In an excellent study, comprehensive analysis of 20 Arabidopsis mutants of genes encoding for enzymes of the lignin pathway was accomplished using a systems approach [107].
To determine which factors act downstream of TORC1 to promote nuclear translocation of Cdc5, we examined mutants of genes involved in TORC1 pathway (Figure 7A).
Additionally, existing mutants of genes identified by microarray analysis to display altered transcription in response to linoleic acid, or regulators of these genes, were tested.
Some of these mutations affect splicing of an IFM specific exon (for example the hdp[3] mutation of troponin I (Tn I) [1]) or coding regions of genes unique to the IFM (such as actin 88F (Act88F)), but some are null or strong hypomorphic mutants of genes that are expressed in all muscle types.
Previous results [8] detect no more than two parameters from the hmg1 and hmg2 mutants with normal distributions on one side at P<0.0001, suggesting that our high-content image-profiling method can detect mutants of genes with major cellular roles even if the mutant show a weak phenotype.
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The mutants of gene replacement lack HSVtk while it is expressed by ectopic transformants.
This success gave us the idea that isolation and utilization of suppressor mutants of gene disruptant encoding essential UAF components such as Rrn5 would lead to construction of mutants with much higher RNA content.
Montes et al. [ 13] showed an increased risk of musculoskeletal infections in Asp299Gly mutants of gene coding TLR4.
At present, we have assayed eclosion rhythms or locomotor activity rhythms for mutants of 69 genes or 14 genes, respectively.
Phenotypic analysis of loss-of-function mutants of these genes showed that they play distinct overlapping and antagonistic roles in development.
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